Some Seed Pre-Treatments on Germination Performance and Seed Quality Improvement in Black Cumin (Nigella sativa L.) Seeds

Authors

DOI:

https://doi.org/10.5281/zenodo.17941407

Keywords:

Black Cumin (Nigella sativa L.), priming, germination, emergence

Abstract

This study was conducted to determine the effects of different pre-sowing treatments on the germination percentage and mean germination time of black cumin (Nigella sativa L.) seeds. The experiment was established in 2025 under laboratory conditions at the Ege University Seed Technology Research and Application Center (TOTEM), using a Completely Randomized Blok Design with three replications. The treatments included GA₃ (500 mg L⁻¹), KH₂PO₄ (2 g L⁻¹), PEG 6000 (300 g L⁻¹), and their combinations (PEG 6000 + GA₃ and PEG 6000 + KH₂PO₄), which were applied as seed priming treatments. Following the treatments, germination tests were carried out in accordance with ISTA rules in terms of the germination percentage and mean germination time. The results showed that there were statistically significant differences among treatments in terms of both germination percentage and mean germination time. The highest germination percentage (88.67%) and the shortest mean germination time (3.99 days) were obtained from the PEG 6000 + KH₂PO₄ treatment. Other effective treatments included PEG 6000 + GA₃ (80.00% – 4.48 days) and KH₂PO₄ alone (80.67% – 7.03 days). In conclusion, KH₂PO₄ and GA₃, particularly when combined with PEG 6000, were found to be effective pre-sowing treatments for promoting faster and more uniform germination in black cumin seeds.

References

Acikgoz, N., Ilker, E., Gokcol, A., 2004. Assessment of biological research on the computer. Ege University, TOTEM, Izmir.

Ahmadian, A., Shiri, Y., Froozandeh, M., 2015. Study of germination and seedling growth of Black cumin (Nigella Sativa L.) treated by hydro and Osmopriming under salt stress conditions. Cercetari Agronomice in Moldova, 48: 69-78.

Ajouri, A., Haben, A., Becker, M., 2004. Seed priming enhances germination and seedling growth of barley under conditions of P and Zn deficiency. Journal of Plant Nutrition and Soil Science, 167: 630–636.

Al-Karaki, G.N., 1998. Response of wheat and barley during germination to seed osmopriming at different water potential. Journal of Agronomy and Crop Science, 181: 229-235.

Amir, R., Cohen, H., Hachem, Y., 2019. Revisiting the attempts to fortify methionine content in plant seeds. Journal of Experimental Botany, 70: 4105–4114.

Anonim, 2020. Tarım ve Orman Bakanlığı Bitkisel Üretim Genel Müdürlüğü Çörek Otu Fizibilite Raporu Ve Yatırımcı Rehberi. (https://www.tarimorman.gov.-tr/SGB/TARYAT/Belgeler/Projeler/%C3%87orek+Otu+Fizibilite+Raporu+ve+Yatirimci+Rehberi.pdf), (Erişim Tarihi: 10.04.2025).

Asaduzzaman, M., Huqe, M.A.S., Uddin, M.N., Hossain, M.A., Haque, M.S., 2021. Seed priming improves germination and early seedling growth in wheat under control and drought conditions. Journal of the Bangladesh Agricultural University, 19: 1-191.

Ceritoğlu, M., Erman, M., Çığ, F., Şahin, S., Abdulkadir, A., 2021. Bitki gelişimi ve stres toleransının geliştirilmesi üzerine sürdürülebilir bir strateji: Priming tekniği. Türkiye Tarımsal Araştırmalar Dergisi, 8(3): 374-389.

Ceylan, A., 1987. Tıbbi Bitkiler II (Uçucu Yağ İçerenler). Ege Üniversitesi Ziraat Fakültesi Yayınları No:481, Bornova, İzmir

Duman, İ., Gökçöl, A., Tuncel, G., Akçalı, G., 2011. Biber tohumlarının kalite özelliklerinin iyileştirilmesinde tohum kaplama uygulamasından yararlanma olanakları, Türkiye IV Tohumculuk Kongresi, 14-17 Haziran, Samsun, s. 11-16.

Durmaz, N., Kara, N., 2023. Çörekotu (Nigella sativa L.)’nda bazı tohum ön uygulamalarının verim ve kalite özelliklerine etkisi. Türk Bilim ve Mühendislik Dergisi, 5(1): 9-14.

Elkoca, E., 2007. Priming: ekim öncesi tohum uygulamaları. Atatürk Üniversitesi Ziraat Fakültesi Dergisi, 38(1): 113-120.

Endes, Z., 2018. Bazı Tohum ön uygulamalarının iki farklı çörek otu türüne ait (Nigella sativa L. ve Nigella damascena L.) Tohumların çimlenme ve çıkış performansı üzerine etkileri. Selcuk Tarim ve Gida Bilimleri Dergisi, 32(1): 29-37.

Fallah, S., Malekzadeh, S., Pessarakli, M., 2017. Seed priming improves seedling emergence and reduces oxidative stress in Nigella sativa under soil moisture stress. Journal of Plant Nutrition, 41(1): 29–40.

Foti, S., Cosentino, S.L., Patane, C., D’Agosta, G.M., 2002. Effect of osmoconditioning upon seed germination of sorghum (Sorghum bicolor (L.) Moench) under low temperatures. Seed Science and Technology, 30: 521-533.

Ghiyasi, M., Amirnia, R., Fazelimanesh, M., 2015. The effect of seed priming with different concentrations of ascorbic acid on germination and vigor of cumin (Cumin cyminum L.) under saline conditions. Thai Journal of Agricultural Science, 48: 179-785.

Ghiyasi, M., Siavash Moghaddam, S., Amirnia, R., 2019. Chemical priming with salt and urea improves germination and seedling growth of black cumin (Nigella sativa L.) under osmotic stress. Journal of Plant Growth Regulation, 38: 1170–1178.

Hartmann, H.T., Kester, D.E., Davies J.F.T., 1990. Plant Propagation Principles and Practices. Prentice Hall, Eaglewood Cliffs.

Hasanuzzaman, M., Nahar, K., Fujita, M., 2012. Plant response to salt stress and role of exogenous protectants to mitigate salt-induced damages. In: P. Ahmad (Ed), Ecophysiology and responses of plants under salt stress. Springer Science + Business Media, Berlin, pp. 25–87.

Işık, S., 2018. Türkiye'de yetiştirilen çörek otu (Nigella sativa L.) tohumlarına ön uygulama (priming) ve biyoteknolojik yöntemler uygulayarak, standardize çörek otu yağının elde edilmesi. Doktora Tezi, Ankara Üniversitesi Biyoteknoloji Enstitüsü, Ankara.

ISTA, 2014. International Rules for Seed Testing. International Seed Testing Association, Zurich, Switzerland.

İlisulu, K., 1992. İlaç ve Baharat Bitkileri, Ankara Üniversitesi Ziraat Fakültesi Yayınları. No: 1256, Ankara.

Kayacetin, F., 2021. Selection of some important species in genus Brassica against drought and salt tolerance by morphological observations on germination and seedling growth parameters. Fresenius Environment Bulletin, 30: 9228–9236.

Kayacetin, F., 2022. Response to direct selection against drought stress in Black cumin (Nigella sativa L.). Evidence-Based Complementary and Alternative Medicine, 2022(1): 6888187.

Larsen, S.U., Andreasen, C., 2004. Light and heavy turfgrass seeds differ in germination percentage and mean germination thermal time. Crop Science, 44(5): 1710–1720.

McDonald, M.B., 2000. Seed Priming. In: M. Black, J.D. Bewley (Eds) Seed Technology and Its Biological Basis. Sheffield Academic Press, Sheffield, UK, pp. 287–325.

Mirmazloum, I., Kiss, A., Erdelyi, E., Ladanyi, M., Nemeth, E.Z., Radacsi, P., 2020. The Effect of osmopriming on seed germination and early seedling characteristics of Carum carvi L. Agriculture, 10(4): 94.

Özcan, İ.İ., Arabacı, O., Öğretmen, N., 2014. Bazı adaçayı türlerinde farklı çimlendirme uygulamalarının belirlenmesi. Türk Tarım Gıda Bilim ve Teknoloji Dergisi, 2: 203–207.

Pedersen, L.H., Jorgensen, P.E., Pulsen, I., 1993. Effect of seed vigor and dormancy on field emergence, development and grain yield of winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). Seed Science & Technology, 21(1): 159-178.

Seyyedi, S.M., Kafi, M., Khazaei, H., Mousavi, S.F., 2015. Effect of priming on seed germination and seedling establishment of black cumin (Nigella sativa L.) under salinity stress. Industrial Crops and Products, 74: 545–552.

Steel, R.G.D., Torrie, J.H., 1980. Principles and procedures of statistics: A biometrical approach. McGraw Hill.

Subaşı, Ü., Gülseven, A., 2010. Seed germination studies on rare endemic Salvia smyrnaea. Biological Diversity and Conservation, 1: 126–132.

TÜİK, 2025. Türkiye İstatistik Kurumu. Bitkisel Üretim İstatistikleri. (https://data.tuik.gov.tr), (Erişim Tarihi: 05.07.2025).

Published

2025-12-17

How to Cite

BAGHIRLI, A., ALIYEV, O. ., AYDIN, M. A. ., MEHMET, M., & ÖZTÜRK, G. (2025). Some Seed Pre-Treatments on Germination Performance and Seed Quality Improvement in Black Cumin (Nigella sativa L.) Seeds. Ejons International Journal on Mathematic, Engineering and Natural Sciences, 9(4), 370–378. https://doi.org/10.5281/zenodo.17941407

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