Effect of Plant Growth Regulators on Sweet Potato [Ipomoea batatas (L.) Lam.] Propagation by Meristem Culture
DOI:
https://doi.org/10.5281/zenodo.14235758Abstract
Sweet potato [Ipomoea batatas (L.) Lam.] is a versatile crop. Since traditional production is done with storage roots, storage roots are very susceptible to diseases. Meristem culture is used as an effective method to obtain disease-free genetic stocks in vegetative propagating species. Shoots of storage root from sweet potato genotype were cultured in MS medium with different concentrations of BAP (6-Benzylaminopurine) and 2.4 D (2.4-Dichlorophenoxyacetic acid). The medium of 3.0 mg/l BAP had the highest mean in terms of the number of shoots as 1.5. The medium containing 1.0 mg/l BAP had highest mean in terms of shoot length (10.6 cm), number of roots (5.0), root length (2.9 cm), number of leaves (9.5) and number of internodes (8.5). The derived shoots were sub cultured on media for different concentrations NAA (1-Naphthalenetic acid). The medium containing 0.1 mg/l NAA was successful in terms of shoot length (3.8 cm), number of roots (2.3), root length (2.9 cm) and number of leaves (4.3). Sweet potato [Ipomoea batata (L.) Lam.] shoots obtained by meristem culture under in vitro conditions can be sub cultured. For this reason, genetically stable plants can be obtained by in vitro regeneration of the apical meristem of sweet potato [Ipomoea batatas (L.) Lam.] as well as rapid and disease-free propagation. On the other hand, this can be provided great advantages and convenience in the commercial production of sweet potato varieties.
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