İngilizce Detection and Genetic Variation of Spa and Tst-1 Gene in Clinical Staphylococcus aureus Samples in THI-QAR
Genetic Variation of Staphylococcus aureus Samples in THI-QAR
DOI:
https://doi.org/10.5281/zenodo.10479022Keywords:
UTI, Staphylococcus aureus, Spa,Tsst-1Abstract
Urinary tract infection (UTI) is one of the most common infectious diseases in humans. Staphylococcus aureus, a gram-positive bacterium, is one of the most common causes of UTI. In this study, it was aimed to investigate the presence and genetic variation of Staphylococcal protein A (spa) and toxic shock syndrome toxin-1 (Tsst-1) gene regions by determining the distribution of S. aureus in urine samples taken from UTI patients in Thi-qar province. Various biochemical methods and API-20 and Vitek2 compact systems were used for the identification of S. aureus. Fourteen different antibiotics, which are frequently used in the clinic, were used to determine the antibiotic susceptibility of the strains. PCR technique was used to determine the presence of spa and tsst-1 genes of S. aureus strains isolated from UTI patients. Genetic variation of strains was determined by DNA sequencing analysis method. In the study, S. aureus was found to be positive in 133 (66.5%) of the urine samples taken from 200 patients with UTI. The highest resistance rates in antibiotic susceptibility of the strains were determined as Ampicillin (100%), Cefoxitin (97.7%), Tetracycline (76.6%), Clindamycin (71.4%) and Trimethoprim-Sulphamethoxazole (54.1%), while Erythromycin (43.6%) and Doxycycline (4%). 42.8) was determined. On the other hand, the strains tested showed a high degree of susceptibility to Vancomycin and Novobiocin. All S. aureus isolates (n=55) were amplified with a 100% positive reaction against the spa gene, while the presence of the tsst-1 gene was determined at 20%. In DNA sequencing analysis, the percentage of genetic variation in the spa gene in native S. aureus isolates was found to be between 0.84-1.05%, while the genetic variation in the tsst-1 gene was found to be 0.23%. As a result of the study, it was seen that there was a significant increase in the prevalence of S. aureus in Iraq, and the genetic variation in the spa and tsst-1 gene was low.
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