EFFECTS OF DIFFERENT PLANT GROWTH REGULATOR CONCENTRATIONS ROOTING OF SP-2 (PRUNUS SPINOSA) CLONAL CANDIDATE ROOTSTOCK IN VITRO CONDITIONS

Abstract

Rooting possibilities In vitro conditions with applied plant growth regulators concentration of SP-2 (Prunus spinosa), candidate rootstock that obtained by in vitro regenerating programm was determined in this study at 2017 year. Medium, and this medium added six different plant growth regulator combinations MSK-1(0.5 mg.l-1 IBA), MSK-2(1 mg.l-1 IBA), MSK-3(0.1 mg.l-1 IBA, 0.5 mg.l-1 BA), MSK-4(0.1 mg.l-1 IBA, 0.5 mg.l-1 TDZ), MSK-5 (0.5 mg.l-1 IBA, 0.1 mg.l-1 TDZ, 0.05 mg.l-1 NAA), and MSK-0 (normal MS without any hormone) as control was used material. Maximum average shoot length per explant at the MSK-3 medium (79.85 mm) was found. In addition, it was determined that maximum root length per (133.33 mm) and highest root number as 4.83 number of shoot at the control medium MSK-0 was observed in this study. At the end of the study, it was observed that the best rooting value MSK-1 medium and the control medium (MSK- 0) gave very positive results was occurred. On the other hand it was decided that clonal rootstock candidate SP-2 (Prunus spinosa) may be rooting without using any plant growth regulators.