The Effect of Zinc oxide nanoparticles on the gene expression of the biofilm formation genes in Pseudomonas aeruginosa isolated from clinical samples
Effect of Zinc oxide nanoparticles on the gene expression of the biofilm formation genes
DOI:
https://doi.org/10.5281/zenodo.10478921Keywords:
biofilm formation, Pseudomonas aeruginosa, Zinc oxide nanoparticlesAbstract
Background and aim: The use of antibiotics to treat Pseudomonas aeruginosa infections is limited by the bacteria's innate antimicrobial resistance. As a result, research has focused on developing advanced antibacterial agents to eradicate resistant bacterial strains. It has been discovered that various nanoparticles can be used as antibacterial agents. This study evaluated the role of zinc oxide nanoparticles (ZnO NPs) as an anti-biofilm agent.
Methods: We used six potent biofilm producer isolates of P. aeruginosa, which have two biofilm genes, to perform a quantitative PCR reaction experiment. These isolates were chosen because they have different sub-MIC values for ZnO-np. A quantitative RT-PCR assay was used to analyze the mRNA expression of biofilm genes in treated and untreated samples of bacterial growth with ZnO-np by using the concentration below the MIC dose for each sample.
Results: The results revealed a significant down-regulation in biofilm genes in the presence of ZnO-np. Correlation analysis was used to analyze the results, which included all the tested isolates before and after treatment with ZnO-np. This analysis showed a significant positive correlation between gene expression of lasI, and rhlI genes and biofilm formation. These findings suggest that ZnO NPs could potentially treat P. aeruginosa infections. However, further research is needed to confirm these findings and to determine the optimal dose and concentration of ZnO NPs for treating P. aeruginosa infections.
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